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polyclonal antibody against arpc2  (Aviva Systems)


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    Structured Review

    Aviva Systems polyclonal antibody against arpc2
    List of VSMC proteins up or downregulated by H 2 O 2 treatment in a Nox1-dependent manner.
    Polyclonal Antibody Against Arpc2, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+antibody+against+arpc2/ARPC2+Antibody+(OALA02841)/pmc03821612-106-14-18
    Average 86 stars, based on 1 article reviews
    polyclonal antibody against arpc2 - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Proteomic Analysis Identifies an NADPH Oxidase 1 (Nox1)-Mediated Role for Actin-Related Protein 2/3 Complex Subunit 2 (ARPC2) in Promoting Smooth Muscle Cell Migration"

    Article Title: Proteomic Analysis Identifies an NADPH Oxidase 1 (Nox1)-Mediated Role for Actin-Related Protein 2/3 Complex Subunit 2 (ARPC2) in Promoting Smooth Muscle Cell Migration

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms141020220

    List of VSMC proteins up or downregulated by H 2 O 2 treatment in a Nox1-dependent manner.
    Figure Legend Snippet: List of VSMC proteins up or downregulated by H 2 O 2 treatment in a Nox1-dependent manner.

    Techniques Used: Molecular Weight

    Upregulation of ARPC2 protein expression in VSMCs via Nox1. Nox1 and Scrmb siRNA-treated VSMC were incubated with vehicle or 50 μM H 2 O 2 for 3 h. VSMC lysates were subjected to Western blot and probed with a polyclonal antibody against ARPC2 (Aviva Systems Biology) and β-actin (Santa Cruz Biotechnology). ( A ) Representative Western blot; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.
    Figure Legend Snippet: Upregulation of ARPC2 protein expression in VSMCs via Nox1. Nox1 and Scrmb siRNA-treated VSMC were incubated with vehicle or 50 μM H 2 O 2 for 3 h. VSMC lysates were subjected to Western blot and probed with a polyclonal antibody against ARPC2 (Aviva Systems Biology) and β-actin (Santa Cruz Biotechnology). ( A ) Representative Western blot; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.

    Techniques Used: Expressing, Incubation, Western Blot

    H 2 O 2 stimulates VSMC migration via ARPC2. ARPC2 and Scrmb siRNA-transfected VSMC were incubated with vehicle or H 2 O 2 (50 μM) for 24 h. Cell migration was determined by wounding of VSMC monolayers. Images were captured at 0 and 24 h. ( A ) Representative Western blot showing ARPC2 and β-actin expression in Scrmb- and ARPC2-siRNA-treated VSMC. Images are representative of three independent experiments with similar results; ( B ) Representative images of wound healing at 0 and 24 h after scratch are shown (original magnification, ×5). Dashed lines denote the approximate edge of the wound at 0 and 24 h time points; ( C ) Quantitative assessment of VSMC migration ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.
    Figure Legend Snippet: H 2 O 2 stimulates VSMC migration via ARPC2. ARPC2 and Scrmb siRNA-transfected VSMC were incubated with vehicle or H 2 O 2 (50 μM) for 24 h. Cell migration was determined by wounding of VSMC monolayers. Images were captured at 0 and 24 h. ( A ) Representative Western blot showing ARPC2 and β-actin expression in Scrmb- and ARPC2-siRNA-treated VSMC. Images are representative of three independent experiments with similar results; ( B ) Representative images of wound healing at 0 and 24 h after scratch are shown (original magnification, ×5). Dashed lines denote the approximate edge of the wound at 0 and 24 h time points; ( C ) Quantitative assessment of VSMC migration ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.

    Techniques Used: Migration, Transfection, Incubation, Western Blot, Expressing

    Inhibition of p38 MAPK pathway attenuates H 2 O 2 -induced ARPC2 expression. VSMC were pre-treated with the p38 MAPK inhibitor (p38i) SB203580 (1 μM, 1 h, Millipore), followed by treatment with vehicle or H 2 O 2 (50 μM) for 3 h. VSMC lysates were subjected to Western blot and probed with polyclonal antibodies against ARPC2 and β-actin. ( A ) Representative Western blot showing ARPC2 and β-actin expression in control- and p38i-treated VSMC incubated with vehicle or H 2 O 2 ; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 4). Data represent the mean ± SEM. * p < 0.05 vs . Control + vehicle treatment. # p < 0.05 vs . Control + H 2 O 2 treatment.
    Figure Legend Snippet: Inhibition of p38 MAPK pathway attenuates H 2 O 2 -induced ARPC2 expression. VSMC were pre-treated with the p38 MAPK inhibitor (p38i) SB203580 (1 μM, 1 h, Millipore), followed by treatment with vehicle or H 2 O 2 (50 μM) for 3 h. VSMC lysates were subjected to Western blot and probed with polyclonal antibodies against ARPC2 and β-actin. ( A ) Representative Western blot showing ARPC2 and β-actin expression in control- and p38i-treated VSMC incubated with vehicle or H 2 O 2 ; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 4). Data represent the mean ± SEM. * p < 0.05 vs . Control + vehicle treatment. # p < 0.05 vs . Control + H 2 O 2 treatment.

    Techniques Used: Inhibition, Expressing, Western Blot, Control, Incubation

    Related Articles

    Transfection:

    Article Title: Proteomic Analysis Identifies an NADPH Oxidase 1 (Nox1)-Mediated Role for Actin-Related Protein 2/3 Complex Subunit 2 (ARPC2) in Promoting Smooth Muscle Cell Migration
    Article Snippet: .. Seventy two hours after transfection, VSMC lysates were subjected to SDS-PAGE/Western blot with a polyclonal antibody against ARPC2 (Aviva Systems Biology, San Diego, CA, USA), total p38 MAPK (Cell Signaling) and β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) and monoclonal antibody against phospho-p38 MAPK (Cell Signaling). .. Membranes were then incubated with the appropriate secondary antibodies (Li-Cor Biotechnology, Lincoln, NE, USA).



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    polyclonal antibody against arpc2  (Aviva Systems)
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    Aviva Systems polyclonal antibody against arpc2
    List of VSMC proteins up or downregulated by H 2 O 2 treatment in a Nox1-dependent manner.
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    polyclonal antibody against p34 (arpc2)  (Upstate Biotechnology Inc)
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    Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, <t>p34</t> and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.
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    Image Search Results


    List of VSMC proteins up or downregulated by H 2 O 2 treatment in a Nox1-dependent manner.

    Journal: International Journal of Molecular Sciences

    Article Title: Proteomic Analysis Identifies an NADPH Oxidase 1 (Nox1)-Mediated Role for Actin-Related Protein 2/3 Complex Subunit 2 (ARPC2) in Promoting Smooth Muscle Cell Migration

    doi: 10.3390/ijms141020220

    Figure Lengend Snippet: List of VSMC proteins up or downregulated by H 2 O 2 treatment in a Nox1-dependent manner.

    Article Snippet: Seventy two hours after transfection, VSMC lysates were subjected to SDS-PAGE/Western blot with a polyclonal antibody against ARPC2 (Aviva Systems Biology, San Diego, CA, USA), total p38 MAPK (Cell Signaling) and β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) and monoclonal antibody against phospho-p38 MAPK (Cell Signaling).

    Techniques: Molecular Weight

    Upregulation of ARPC2 protein expression in VSMCs via Nox1. Nox1 and Scrmb siRNA-treated VSMC were incubated with vehicle or 50 μM H 2 O 2 for 3 h. VSMC lysates were subjected to Western blot and probed with a polyclonal antibody against ARPC2 (Aviva Systems Biology) and β-actin (Santa Cruz Biotechnology). ( A ) Representative Western blot; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.

    Journal: International Journal of Molecular Sciences

    Article Title: Proteomic Analysis Identifies an NADPH Oxidase 1 (Nox1)-Mediated Role for Actin-Related Protein 2/3 Complex Subunit 2 (ARPC2) in Promoting Smooth Muscle Cell Migration

    doi: 10.3390/ijms141020220

    Figure Lengend Snippet: Upregulation of ARPC2 protein expression in VSMCs via Nox1. Nox1 and Scrmb siRNA-treated VSMC were incubated with vehicle or 50 μM H 2 O 2 for 3 h. VSMC lysates were subjected to Western blot and probed with a polyclonal antibody against ARPC2 (Aviva Systems Biology) and β-actin (Santa Cruz Biotechnology). ( A ) Representative Western blot; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.

    Article Snippet: Seventy two hours after transfection, VSMC lysates were subjected to SDS-PAGE/Western blot with a polyclonal antibody against ARPC2 (Aviva Systems Biology, San Diego, CA, USA), total p38 MAPK (Cell Signaling) and β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) and monoclonal antibody against phospho-p38 MAPK (Cell Signaling).

    Techniques: Expressing, Incubation, Western Blot

    H 2 O 2 stimulates VSMC migration via ARPC2. ARPC2 and Scrmb siRNA-transfected VSMC were incubated with vehicle or H 2 O 2 (50 μM) for 24 h. Cell migration was determined by wounding of VSMC monolayers. Images were captured at 0 and 24 h. ( A ) Representative Western blot showing ARPC2 and β-actin expression in Scrmb- and ARPC2-siRNA-treated VSMC. Images are representative of three independent experiments with similar results; ( B ) Representative images of wound healing at 0 and 24 h after scratch are shown (original magnification, ×5). Dashed lines denote the approximate edge of the wound at 0 and 24 h time points; ( C ) Quantitative assessment of VSMC migration ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.

    Journal: International Journal of Molecular Sciences

    Article Title: Proteomic Analysis Identifies an NADPH Oxidase 1 (Nox1)-Mediated Role for Actin-Related Protein 2/3 Complex Subunit 2 (ARPC2) in Promoting Smooth Muscle Cell Migration

    doi: 10.3390/ijms141020220

    Figure Lengend Snippet: H 2 O 2 stimulates VSMC migration via ARPC2. ARPC2 and Scrmb siRNA-transfected VSMC were incubated with vehicle or H 2 O 2 (50 μM) for 24 h. Cell migration was determined by wounding of VSMC monolayers. Images were captured at 0 and 24 h. ( A ) Representative Western blot showing ARPC2 and β-actin expression in Scrmb- and ARPC2-siRNA-treated VSMC. Images are representative of three independent experiments with similar results; ( B ) Representative images of wound healing at 0 and 24 h after scratch are shown (original magnification, ×5). Dashed lines denote the approximate edge of the wound at 0 and 24 h time points; ( C ) Quantitative assessment of VSMC migration ( n = 6). Data represent the mean ± SEM. * p < 0.05 vs . Scrmb siRNA + vehicle treatment; # p < 0.05 vs . Scrmb siRNA + H 2 O 2 treatment.

    Article Snippet: Seventy two hours after transfection, VSMC lysates were subjected to SDS-PAGE/Western blot with a polyclonal antibody against ARPC2 (Aviva Systems Biology, San Diego, CA, USA), total p38 MAPK (Cell Signaling) and β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) and monoclonal antibody against phospho-p38 MAPK (Cell Signaling).

    Techniques: Migration, Transfection, Incubation, Western Blot, Expressing

    Inhibition of p38 MAPK pathway attenuates H 2 O 2 -induced ARPC2 expression. VSMC were pre-treated with the p38 MAPK inhibitor (p38i) SB203580 (1 μM, 1 h, Millipore), followed by treatment with vehicle or H 2 O 2 (50 μM) for 3 h. VSMC lysates were subjected to Western blot and probed with polyclonal antibodies against ARPC2 and β-actin. ( A ) Representative Western blot showing ARPC2 and β-actin expression in control- and p38i-treated VSMC incubated with vehicle or H 2 O 2 ; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 4). Data represent the mean ± SEM. * p < 0.05 vs . Control + vehicle treatment. # p < 0.05 vs . Control + H 2 O 2 treatment.

    Journal: International Journal of Molecular Sciences

    Article Title: Proteomic Analysis Identifies an NADPH Oxidase 1 (Nox1)-Mediated Role for Actin-Related Protein 2/3 Complex Subunit 2 (ARPC2) in Promoting Smooth Muscle Cell Migration

    doi: 10.3390/ijms141020220

    Figure Lengend Snippet: Inhibition of p38 MAPK pathway attenuates H 2 O 2 -induced ARPC2 expression. VSMC were pre-treated with the p38 MAPK inhibitor (p38i) SB203580 (1 μM, 1 h, Millipore), followed by treatment with vehicle or H 2 O 2 (50 μM) for 3 h. VSMC lysates were subjected to Western blot and probed with polyclonal antibodies against ARPC2 and β-actin. ( A ) Representative Western blot showing ARPC2 and β-actin expression in control- and p38i-treated VSMC incubated with vehicle or H 2 O 2 ; ( B ) Bar graphs representing averaged optical density data expressed as a ratio of ARPC2 to β-actin ( n = 4). Data represent the mean ± SEM. * p < 0.05 vs . Control + vehicle treatment. # p < 0.05 vs . Control + H 2 O 2 treatment.

    Article Snippet: Seventy two hours after transfection, VSMC lysates were subjected to SDS-PAGE/Western blot with a polyclonal antibody against ARPC2 (Aviva Systems Biology, San Diego, CA, USA), total p38 MAPK (Cell Signaling) and β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) and monoclonal antibody against phospho-p38 MAPK (Cell Signaling).

    Techniques: Inhibition, Expressing, Western Blot, Control, Incubation

    Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, p34 and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.

    Journal: BMC Cell Biology

    Article Title: Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway

    doi: 10.1186/1471-2121-9-42

    Figure Lengend Snippet: Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, p34 and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.

    Article Snippet: Polyclonal antibody against p34 (ARPC2) was from Upstate Biotechnology.

    Techniques: Transfection, Western Blot, Flow Cytometry, Knockdown